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Title: |
Stoichiometric homeostasis of N:P ratio drives species-specific symbiotic N fixation inhibition under N addition |
| First author: |
Li, Qiang |
| Abstract: |
IntroductionSymbiotic N fixation inhibition induced by N supply to legumes is potentially regulated by the relative N and P availability in soil. However, the specific responses of different legume species to changes in N:P availability remain unclear, and must be better understood to optimize symbiotic N fixation inputs under N enrichment. This study investigated mechanisms by which soil N and P supply influence the symbiotic N fixation of eight legume species, to quantify the inter-specific differences, and to demonstrate how these differences can be determined by the stoichiometric homeostasis in N:P ratios (H-N:P). MethodsEight herbaceous legume species were grown separately in outdoor pots and treated with either no fertilizer (control), N fertilizer (14 g N m(-2)), P fertilizer (3.5 g P m(-2)) or both N and P fertilizer. Plant nutrients, stoichiometric characteristics, root biomass, non-structural carbohydrates (NSC), rhizosphere chemistry, P mobilization, root nodulation and symbiotic N fixation were measured. ResultsN addition enhanced rhizosphere P mobilization but drove a loss of root biomass and root NSC via exudation of P mobilization compound (organic acid), especially so in treatments without P addition. N addition also induced a 2-14% or 14-36% decline in symbiotic N fixation per plant biomass by legumes in treatments with or without P addition, as a result of decreasing root biomass and root NSC. The changes in symbiotic N fixation were positively correlated with stoichiometric homeostasis of N:P ratios in intact plants without root nodules, regardless of P additions. DiscussionThis study indicates that N addition can induce relative P limitations for growth, which can stimulate rhizosphere P mobilization at the expense of root biomass and carbohydrate concentrations, reducing symbiotic N fixation in legumes. Legume species that had less changes in plant N:P ratio, such as Lespedeza daurica and Medicago varia maintained symbiotic N fixation to a greater extent under N addition.
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| Contact the author: |
Li, Qiang; Huang, Yingxin;Denton, Matthew D. D.;Wei, Jian |
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| PubYear: |
2023 |
| Volume: |
14 |
| Unit code: |
131322 |
| Publication name: |
FRONTIERS IN PLANT SCIENCE |
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Li,Q(Li,Qiang)[1],[2];Philp,J(Philp,Joshua)[3];Denton,MD(Denton,MatthewD.D.)[3];Huang,YX(Huang,Yingxin)[1],[2];Wei,J(Wei,Jian)[4];Sun,HJ(Sun,Huijuan)[1],[2];Li,Y(Li,Yang)[2],[5];Zhao,Qian[4] |
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